Characterization of Four Covalently-Linked Yeast Cytochrome c/Cytochrome c Peroxidase Complexes: Evidence for Electrostatic Interaction between Bound Cytochrome c Molecules
نویسندگان
چکیده
منابع مشابه
Crystal structure and characterization of a cytochrome c peroxidase-cytochrome c site-specific cross-link.
A specific covalently cross-linked complex between redox partners yeast cytochrome c peroxidase (CCP) and cytochrome c (cyt. c) has been made by engineering cysteines into CCP and cyt. c that form an intermolecular disulfide bond in high yield. The crystal structure of the cross-linked complex has been solved to 1.88-A resolution and closely resembles the structure of the noncovalent complex [P...
متن کاملInvestigation of electron tunneling between cytochrome c peroxidase and cytochrome c.
The nature of electron transfer between the bound complex cytochrome c and cytochrome c peroxidase has been investigated. Experimental verification of the predicted charge-transfer band provides evidence of electron tunneling as the mechanism of transfer between these molecules in solution at room temperature. The measured transfer distance is congruent to 7 angstroms between heme edges, which ...
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Apoproteins of cytochrome c peroxidase, horseradish peroxidase, and sperm whale myoglobin were recombined with manganese complexes of proto-, hemato-, meso-, and deuteroporphyrins to form manganese porphyrin-protein complexes. These complexes were purified by column chromatography. All the manganese porphyrin-containing cytochrome c peroxidases were crystallized. Light absorption maxima of mang...
متن کاملMagnetic susceptibility measurements of cytochrome c peroxidase and its complexes.
A highly purified preparation of cytochrome c peroxidase was prepared by reconstituting the enzyme from the apoprotein and protohemin. Paramagnetic susceptibilities of the enzyme and of its complexes with cyanide, azide, cyanate, and fluoride were measured at temperatures from 77” K to 273” K. The susceptibilities of the cyanide and fluoride complexes obeyed Curie’s law over the entire temperat...
متن کاملComplex-formation between cytochrome c and cytochrome c peroxidase. Equilibrium and titration studies.
1. Physical studies of complex-formation between cytochrome c and yeast peroxidase are consistent with kinetic predictions that these complexes participate in the catalytic activity of yeast peroxidase towards ferrocytochrome c. Enzyme-ferricytochrome c complexes have been detected both by the analytical ultracentrifuge and by column chromatography, whereas an enzyme-ferrocytochrome c complex w...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2006
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi061662p